| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.056131 |
| Chromosome: | chromosome 16 |
| Location: | 1292814 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651450 | (1 of 3) PTHR13367:SF8 - OTU DOMAIN-CONTAINING PROTEIN 7B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGCAGCGTGTAGGTGACTGTGCCTGTT |
| Internal bar code: | ACCTCAGATGACGATACGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 99.7677 |
| LEAP-Seq n confirming: | 3006 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTGGCAGCTTCAACTTC |
| Suggested primer 2: | ACGGAGATCAGCTGGAAATG |