Insertion junction: LMJ.RY0402.056140_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.056140
Chromosome:chromosome 3
Location:881158
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g146607 (1 of 173) IPR029058 - Alpha/Beta hydrolase fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGACCCAGTCAATCGGCGGAAATGAGC
Internal bar code:GAGCTGCGGGTATCCCTAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:358
LEAP-Seq percent confirming:99.796
LEAP-Seq n confirming:1957
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTAGCGAAGCTATTGTT
Suggested primer 2:TGTAAACGCACGCTCTGACT