| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056165 |
| Chromosome: | chromosome 17 |
| Location: | 166400 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697334 | (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGTGCTGGAATAATGCCCAGCCTGTC |
| Internal bar code: | TCGAGGACCAGCATCGGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 942 |
| LEAP-Seq percent confirming: | 99.4125 |
| LEAP-Seq n confirming: | 846 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAATTTTAGGCCCGTAGC |
| Suggested primer 2: | TAAAAACGTGCTATGCTGCG |