| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.056177 |
| Chromosome: | chromosome 1 |
| Location: | 3919077 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025500 | (1 of 1) PTHR19959//PTHR19959:SF135 - KINESIN LIGHT CHAIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGGCAAACACTGCGAACCGGAACGGA |
| Internal bar code: | GGGATGCTCCCGGGGGCTGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 99.7668 |
| LEAP-Seq n confirming: | 5133 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGGGTACAACTTTGAGC |
| Suggested primer 2: | ACCTTACGACATTTCCAGCG |