| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.056278 |
| Chromosome: | chromosome 3 |
| Location: | 5682390 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187700 | HEL19 | (1 of 1) PTHR24031//PTHR24031:SF252 - RNA HELICASE // SUBFAMILY NOT NAMED; DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCAGCCGCTTCCCCCGGTGCCCCTGCC |
| Internal bar code: | GCCTCCATGGAGGGAGGATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 99.3197 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACCTCATGGGTCTTGGC |
| Suggested primer 2: | ACTTGCTGTCGGAGCTGTTT |