| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.056284 |
| Chromosome: | chromosome 8 |
| Location: | 1461597 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364550 | (1 of 3) K10587 - ubiquitin-protein ligase E3 A (UBE3A, E6AP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTAAACACATATGGTATGCTCACAAAA |
| Internal bar code: | GGCATTTGAGTTAGTAAGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 72 |
| LEAP-Seq percent confirming: | 99.7136 |
| LEAP-Seq n confirming: | 26459 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTATACTCGGCGAGAC |
| Suggested primer 2: | ACCCGTAACCGTAACTGCTG |