Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.056289 |
Chromosome: | chromosome_14 |
Location: | 2454739 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g624950 | DHC10,IDA2,DYH1B | Flagellar inner arm dynein 1 heavy chain beta | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACTGGATGGCGTTCTCAATCTGGCGCGCCA |
Internal bar code: | ATGACCAGGCCGGGGTGATGAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 828 |
LEAP-Seq percent confirming: | 98.9501 |
LEAP-Seq n confirming: | 1131 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCGTAGGTGTGGAAGTCAT |
Suggested primer 2: | TCTGAGGACACGCAATCAAG |