Insertion junction: LMJ.RY0402.056294_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.056294
Chromosome:chromosome 17
Location:3193798
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g722050 (1 of 1) K19202 - histone deacetylase complex subunit SAP30 (SAP30) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAAGCTGGACGTGAACTCGCTGAAGCGG
Internal bar code:CACGGTGGCTTAACTGGGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:931
LEAP-Seq percent confirming:98.6239
LEAP-Seq n confirming:215
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGTGAACAGATGCTGGC
Suggested primer 2:CCACATACGCTGCAGACACT