| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056296 |
| Chromosome: | chromosome 13 |
| Location: | 5189985 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607900 | CPLD68 | Conserved in the Plant Lineage and Diatoms; (1 of 96) IPR019734 - Tetratricopeptide repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAGGCGGCCAGGGCGGCCGCATAACTG |
| Internal bar code: | CGCCTGCGTCCCTACGGCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 99.6947 |
| LEAP-Seq n confirming: | 653 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATGTGACTCATTGCAGC |
| Suggested primer 2: | GGAGGACGTATACAAGCCCA |