| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056340 |
| Chromosome: | scaffold 18 |
| Location: | 152376 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749197 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTGGGGGGTGTCGACAGCTAGGTGTTT |
| Internal bar code: | GACATCGAGAACGCGTGTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 99.5042 |
| LEAP-Seq n confirming: | 4817 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTACGGTGTGAACACGA |
| Suggested primer 2: | TCAGGAGACTGCGAACACAC |