| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056347 |
| Chromosome: | chromosome 16 |
| Location: | 4813746 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686250 | (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCCAGCACCAAAATGAACACCAGAAGC |
| Internal bar code: | CGATGAAGAATTATAGATGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 39.3838 |
| LEAP-Seq n confirming: | 1534 |
| LEAP-Seq n nonconfirming: | 2361 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAATGCCATACCTTCGCC |
| Suggested primer 2: | CAGCGGTAACTGTGGCTGTA |