Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.056398 |
Chromosome: | chromosome_14 |
Location: | 3617329 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g631200 | DII6,IC97,FAP94 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATAAAGTCCGAAGTCGAAGGTGCATAGCTC |
Internal bar code: | TTGAATGAAGATCGCCCGGAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 893 |
LEAP-Seq percent confirming: | 98.4375 |
LEAP-Seq n confirming: | 63 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACACACGCCATTTGTGGAT |
Suggested primer 2: | TCTCTTCTCTTCTCAGCCGC |