| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.056439 |
| Chromosome: | chromosome 5 |
| Location: | 411241 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232850 | COX17 | Cytochrome c oxidase assembly protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGATGGGACCTGTCTGGCCTTAACGA |
| Internal bar code: | TGTCCTTGCTCGTCTCACTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 99.8379 |
| LEAP-Seq n confirming: | 3079 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACTGTCAGCTCCGCATA |
| Suggested primer 2: | AAGACCTGCTGGGTACGATG |