| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.056459 |
| Chromosome: | chromosome 12 |
| Location: | 9423579 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539900 | (1 of 1) K00166 - 2-oxoisovalerate dehydrogenase E1 component alpha subunit (BCKDHA, bkdA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGCACCCTACGGGACTTCATCAACCAG |
| Internal bar code: | CCTCCTGTAGTTCATCGGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 66.8449 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATACTTGTCGGTGGACG |
| Suggested primer 2: | TACATACGGTAGTGCGAGCG |