| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056475 |
| Chromosome: | chromosome 17 |
| Location: | 4053654 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729200 | (1 of 1) PF04678 - Mitochondrial calcium uniporter (MCU) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTCGGTGCCCGCGCCGCCACGCACTGC |
| Internal bar code: | GTGTCCCATGAACGAAGGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 97.8806 |
| LEAP-Seq n confirming: | 3279 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACTTTGGACTGGGGAGT |
| Suggested primer 2: | GCCGTACCAGGTGAGGTAGA |