Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.056525 |
Chromosome: | chromosome_8 |
Location: | 3804594 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g377350 | FAP121,FAP180,ADK5 | Adenylate kinase; Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCCGGCTGGCGCCCTGCGCAGGGTGGGTG |
Internal bar code: | TGCGCGCGATCATACCCGTTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 40 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 16 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGGCGTGTTGTAGTGGTG |
Suggested primer 2: | AGTCGAGTTGCGCTACAGGT |