Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.056527 |
Chromosome: | chromosome 16 |
Location: | 6227818 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g675050 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACTCGGAGCTATGAACCTTTTGTGCG |
Internal bar code: | CACTCCGGGTTTCGTTTTTCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 469 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 218 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGATCGCTGAGGTCAACA |
Suggested primer 2: | CCTTACTGGACACCCATGCT |