| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056558 |
| Chromosome: | chromosome 3 |
| Location: | 8949981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g208833 | (1 of 1) K10896 - fanconi anemia group M protein (FANCM) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGCAGAAGAAGGACCAGTGGCTGGTGC |
| Internal bar code: | GCCTCAACTGTTACGGGAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 99.4223 |
| LEAP-Seq n confirming: | 1549 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGATGCAGTCATGGCAC |
| Suggested primer 2: | ACTACGGTATGCCAAGTCGG |