| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.056571 |
| Chromosome: | chromosome 1 |
| Location: | 652705 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003516 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACGTCGCCCATGCACCGCATCCCTTG |
| Internal bar code: | CCGGTCGCCACACGTCGACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 98.682 |
| LEAP-Seq n confirming: | 3594 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCATCTCTCATTCTCGC |
| Suggested primer 2: | ACCGGCGTATCAAAAGACAC |