Insertion junction: LMJ.RY0402.056578_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.056578
Chromosome:chromosome 7
Location:5379989
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g350100 (1 of 1) IPR000104//IPR006311//IPR017956//IPR026741//IPR026937//IPR027417 - Antifreeze protein, type I // Twin-arginine translocation pathway, signal sequence // AT hook, DNA-binding motif // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGGGAACAAAGCGGTTGCCACATAGCA
Internal bar code:TGTGCACGGGAATTTGTCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAAACTAAACCAAAGCCA
Suggested primer 2:CTCTGGGATTCCTTGTTGGA