Insertion junction: LMJ.RY0402.056605_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.056605
Chromosome:chromosome 1
Location:6822165
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g049200 (1 of 4) PTHR10334//PTHR10334:SF224 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCTAGGAAAGTGCGCCACCAGCCATGC
Internal bar code:GTGTGCACCAAACGGACAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:993
LEAP-Seq percent confirming:99.0463
LEAP-Seq n confirming:1454
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GATGCCTTACTGCTCAAGCC
Suggested primer 2:TACAGTGGACAAACGGGACA