Insertion junction: LMJ.RY0402.056606_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.056606
Chromosome:chromosome 2
Location:3351509
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095095 PHC12 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 12 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGTCAAAAGGAGTGTGCAGGAGCTCGT
Internal bar code:CGGCGCTTCTGACAGTTCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:93.3057
LEAP-Seq n confirming:1352
LEAP-Seq n nonconfirming:97
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACCTGCTGATTAACTGA
Suggested primer 2:GTGTGGTGGTGTCGATGAAG