Insertion junction: LMJ.RY0402.056618_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.056618
Chromosome:chromosome 3
Location:8252817
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g199500 FAP129 Flagellar Associated Protein 129 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGCGTTGAACAGCCCGTTGTCAGACAC
Internal bar code:AGTGCGTGCCTTAGGCGTTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:99.1889
LEAP-Seq n confirming:2935
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCTCTTGCCCGAATGTCT
Suggested primer 2:GATCCAGCGTCATGGTGTC