| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.056618 |
| Chromosome: | chromosome 3 |
| Location: | 8252826 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199500 | FAP129 | Flagellar Associated Protein 129 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAGGCCAAGAGCCAGGGGGCGCTGGGC |
| Internal bar code: | GGTTACCGTGGGCCTCCCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1258 |
| LEAP-Seq percent confirming: | 99.7418 |
| LEAP-Seq n confirming: | 11977 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTCTTGCCCGAATGTCT |
| Suggested primer 2: | GATCCAGCGTCATGGTGTC |