Insertion junction: LMJ.RY0402.056668_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.056668
Chromosome:chromosome 2
Location:6917662
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g119850 PDE9 (1 of 3) 3.1.4.17//3.1.4.53 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase // 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCACGTGTCTTCAGCTTTCCGTCATCGC
Internal bar code:CGCGGTGTTAGGGATTGTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:444
LEAP-Seq percent confirming:97.9567
LEAP-Seq n confirming:1630
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GAATTGCTGTGCGTCTTTCA
Suggested primer 2:TGAAGCAGCACTTCACCATC