| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056773 |
| Chromosome: | chromosome 10 |
| Location: | 1681562 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430100 | ELG22 | Exostosin-like glycosyltransferase 22; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCCAGCAGGCGCCAGAAGGTCAGATGC |
| Internal bar code: | GTGAAGTTTCTTCCAACTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 96.0101 |
| LEAP-Seq n confirming: | 47910 |
| LEAP-Seq n nonconfirming: | 1991 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGAATGACACACGGTAA |
| Suggested primer 2: | GTTTCATGCGCTTCTGTCAA |