Insertion junction: LMJ.RY0402.056779_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.056779
Chromosome:chromosome 4
Location:3037575
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g225176 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGGACAGGCGTTTCCACAGCCAGCGCCG
Internal bar code:CCAGGGCCCCGCACACTGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:865
LEAP-Seq percent confirming:97.8892
LEAP-Seq n confirming:742
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GACGATGCGGTGTAATTGTG
Suggested primer 2:CAAGGAGTCTGTGATGGGGT