Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.056804 |
Chromosome: | chromosome 12 |
Location: | 1820232 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g511950 | (1 of 1) IPR003591//IPR013210 - Leucine-rich repeat, typical subtype // Leucine-rich repeat-containing N-terminal, plant-type | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGAGGAAGAGGGCTGGCGAATGGGCG |
Internal bar code: | ATTGTCGGTGGTGTGCGAACTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1156 |
LEAP-Seq percent confirming: | 99.7387 |
LEAP-Seq n confirming: | 30920 |
LEAP-Seq n nonconfirming: | 81 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTTAACCCCCACTTACCCG |
Suggested primer 2: | TACTGATGAGCAGTGTCGGC |