| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056807 |
| Chromosome: | chromosome 10 |
| Location: | 753647 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422850 | (1 of 5) PTHR11614:SF87 - MONOGLYCERIDE LIPASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCCTCGAACGAATTGCCTCTGTGCTC |
| Internal bar code: | CTTTGGGTGGTGGGAAATTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 99.293 |
| LEAP-Seq n confirming: | 2809 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGGCCACAATAACCAC |
| Suggested primer 2: | GCATGGGTTACCATATTCCG |