| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.056837 |
| Chromosome: | chromosome 8 |
| Location: | 1491859 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364751 | BLZ16 | bZIP transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCCGGGCTGGAAGACGGAGAGGGCTCC |
| Internal bar code: | GCGAAGATCCATCTATTAATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 99.1347 |
| LEAP-Seq n confirming: | 2635 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCTCCGTCTCCTAGCTC |
| Suggested primer 2: | TCACGTACGGCATTTGATGT |