| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.056850 |
| Chromosome: | chromosome 11 |
| Location: | 138636 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467541 | (1 of 3) PTHR13780//PTHR13780:SF49 - AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCAGGCTTGGGGTGTGGGCGCTGGGTGG |
| Internal bar code: | GCTTTTAGTAGCTTCGTAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1372 |
| LEAP-Seq percent confirming: | 99.6804 |
| LEAP-Seq n confirming: | 2807 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGACACATGCAGCGAC |
| Suggested primer 2: | AGGCAGCAGGAGAAAGACAG |