| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.056865 |
| Chromosome: | chromosome 14 |
| Location: | 3188404 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629102 | (1 of 2) PTHR11731//PTHR11731:SF117 - PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED // GLUTAMYL ENDOPEPTIDASE, CHLOROPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTACGTGTGCTGCCGCAGGCATCGCCC |
| Internal bar code: | GTCTCTTCATTCGAATGAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 99.8782 |
| LEAP-Seq n confirming: | 820 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGATCTTGTCAGTGGC |
| Suggested primer 2: | AAAGTGCTTGTTGGGTGTCC |