| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.056867 |
| Chromosome: | chromosome_8 |
| Location: | 1952574 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g367550 | FAP291 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAATGGCAAGAGCGTGTCACTGCACGGTC |
| Internal bar code: | GCGCCAGCGGGAATGGGAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.4167 |
| LEAP-Seq n confirming: | 1193 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTGTGGACTGTGGACT |
| Suggested primer 2: | GAGGCTGCAATGCACATAGA |