| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.056869 |
| Chromosome: | chromosome 14 |
| Location: | 657622 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612226 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGGTCGGTGCGCCCGCCTGCGGGGCT |
| Internal bar code: | GGCACTGGTCGGCTCGGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 90.6421 |
| LEAP-Seq n confirming: | 1327 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCTGCTCACTGCCTACA |
| Suggested primer 2: | CAGTGAGTCCTAGCCAAGCC |