| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.056873 |
| Chromosome: | chromosome 10 |
| Location: | 5268627 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457331 | (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGATGACCCACTGCCCCGCAGGTCCCTG |
| Internal bar code: | CAACTACGGTGGCCGCAGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 98.152 |
| LEAP-Seq n confirming: | 2337 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAAGTCCCATGTCACGCC |
| Suggested primer 2: | ACACGGCGTTGACAATGATA |