| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.056876 |
| Chromosome: | chromosome 17 |
| Location: | 5750731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739150 | CTPA5,TSP5 | (1 of 8) 3.4.21.102 - C-terminal processing peptidase / Tsp protease; putative C-terminal peptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGGCGAGGTATGCACTGAAAACCGCGA |
| Internal bar code: | TAACTGAGCGAGGGTGCAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 99.4624 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGCATATGTGTGTGCC |
| Suggested primer 2: | CACGGAAAGAACGGATTGAT |