| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056884 |
| Chromosome: | chromosome 16 |
| Location: | 5088445 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCGGGGCTCCCGCCAGCTCGGGTCGC |
| Internal bar code: | TCCCGGGGGCGGCCACGTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 98.3174 |
| LEAP-Seq n confirming: | 3389 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCACAGCTTTTCCACGA |
| Suggested primer 2: | CAGACTGTGCAGGATCAGGA |