| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.056915 |
| Chromosome: | chromosome 2 |
| Location: | 3893788 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096150 | MSD1,SODA | (1 of 5) K04564 - superoxide dismutase, Fe-Mn family (SOD2); Mn superoxide dismutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTCGCAGGACAACCCGCTGATGACCGC |
| Internal bar code: | ACCAAAACGAGCTGTATATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACTTCAACGAGGGAAAC |
| Suggested primer 2: | GCACCGATAAGCTGCCTAAG |