| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.056921 |
| Chromosome: | chromosome 2 |
| Location: | 6650633 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117500 | HXK1 | Hexokinase; (1 of 1) K00844 - hexokinase (HK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGTTTTGCGTATGGGAAATGATAATGC |
| Internal bar code: | ACCGGTTTCAATTAGCAGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 98.2558 |
| LEAP-Seq n confirming: | 6422 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACCAGGCGTAGTGTCC |
| Suggested primer 2: | GCCTGAACGTGATCTGGATT |