Insertion junction: LMJ.RY0402.056923_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.056923
Chromosome:chromosome 9
Location:1777905
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396300 PPX1,PPOX (1 of 1) 1.3.3.4 - Protoporphyrinogen oxidase / Protoporphyrinogenase; Protoporphyrinogen oxidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCCGATCCATTCCAACTGTTACTACAC
Internal bar code:AGTCCGAATCGTATCCCACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:740
LEAP-Seq percent confirming:99.6805
LEAP-Seq n confirming:1248
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTCGAGTTGGCATTGTT
Suggested primer 2:GAGCAGGAGGTGGTGCTAAG