Insertion junction: LMJ.RY0402.056965_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.056965
Chromosome:chromosome 3
Location:773335
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g146287 (1 of 6) 3.1.1.1 - Carboxylesterase / Procaine esterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCGGACGCAGCGCCATATTCCTCCCAGT
Internal bar code:GTCTTAGGAATAGGTCCCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:282
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCAATAACCCAGGCCAGTTC
Suggested primer 2:CGCGACCTGTTGTAATAGCA