| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056973 |
| Chromosome: | chromosome 7 |
| Location: | 1197272 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321000 | CYG14 | (1 of 1) PF00211//PF01590 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // GAF domain (GAF); Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACACAATTCCTGTCTTGCGCACACGACA |
| Internal bar code: | AGAACCCGATCGGAGCCCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 97.7073 |
| LEAP-Seq n confirming: | 3452 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGCGAACAGTGTCGAGT |
| Suggested primer 2: | GAGCCGCTGCTTTTATCAAC |