Insertion junction: LMJ.RY0402.056974_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.056974
Chromosome:chromosome 3
Location:8630405
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g203457 (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGTTTTGGGCGTGATCGCTAGGGAATG
Internal bar code:CAGTTATTGCTCCCCCCGCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCAAAAGTGATGCCGTCC
Suggested primer 2:TGCTGTGGGTGTACTGGTGT