Insertion junction: LMJ.RY0402.057015_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.057015
Chromosome:chromosome 2
Location:318447
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g075400 (1 of 6) PTHR15907//PTHR15907:SF21 - FAMILY NOT NAMED // DUF614 FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCATTCTTACGTCGCAACTGATGCCGTG
Internal bar code:GGCGTATCGACTTGCAGTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:821
LEAP-Seq percent confirming:99.4831
LEAP-Seq n confirming:4042
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCTTCCGGACCCTTCAGT
Suggested primer 2:GCTGCGAGGAAGGATTGTAG