| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057028 |
| Chromosome: | chromosome 11 |
| Location: | 2759688 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477200 | IFR1 | Putative 2'-hydroxyisoflavone reductase; (1 of 19) PF01370 - NAD dependent epimerase/dehydratase family (Epimerase) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGACCAAGACTGGGACGGCAAGGAGTGG |
| Internal bar code: | GGGCCGTGCTCCTCAAAATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1154 |
| LEAP-Seq percent confirming: | 99.1886 |
| LEAP-Seq n confirming: | 1956 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAAAGGAACAAGCTCAA |
| Suggested primer 2: | TGAATCAGCAGAGTGTTCGG |