Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.057045 |
Chromosome: | chromosome 3 |
Location: | 8106318 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCCCCATGTTCGCGCGTGCGTATGCTG |
Internal bar code: | ATATAATTATCAGCCAGTCCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 814 |
LEAP-Seq percent confirming: | 99.6313 |
LEAP-Seq n confirming: | 2432 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACTTTCTGAGTGCTTCCC |
Suggested primer 2: | GATCAGGGCAGCTCACTAGG |