Insertion junction: LMJ.RY0402.057045_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.057045
Chromosome:chromosome 7
Location:2950620
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g332851 SELM2,SELENOM,SELM (1 of 1) PTHR13077:SF7 - SELENOPROTEIN M; Selenoprotein M intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAGCGTGGAACCAGGAGGCGACGCTGC
Internal bar code:TGGCGGATGGGTACTAAAGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1115
LEAP-Seq percent confirming:99.6448
LEAP-Seq n confirming:31697
LEAP-Seq n nonconfirming:113
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCAGAATGTCTGTGTGGG
Suggested primer 2:TGTTGTGACCAGGAGATCCA