| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.057053 |
| Chromosome: | chromosome 6 |
| Location: | 8414231 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307012 | (1 of 5) PF13391 - HNH endonuclease (HNH_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACATACCGCAGAGAGAAAGGTGACAGG |
| Internal bar code: | TTGCCTCGTGGTTTGCGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 98.3477 |
| LEAP-Seq n confirming: | 3869 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGTGTGTGTGTGTTGGG |
| Suggested primer 2: | TGAGTGTGAGCCACTTTTGC |