Insertion junction: LMJ.RY0402.057057_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.057057
Chromosome:chromosome 3
Location:6739058
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g197900 (1 of 1) PTHR24375//PTHR24375:SF183 - FAMILY NOT NAMED // C2H2 AND C2HC ZINC FINGERS SUPERFAMILY PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAACAGAGGTGCCCAGAGGTGCCCCAGT
Internal bar code:CTGGTTTTAGGCTGCGCGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1159
LEAP-Seq percent confirming:99.0444
LEAP-Seq n confirming:4457
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGTGGCATAGTATCCGGC
Suggested primer 2:TCGTGTTCAACAAGGAGACG