| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057058 |
| Chromosome: | chromosome 12 |
| Location: | 2101796 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509250 | EYE2 | Eyespot assembly protein 2; (1 of 1) PTHR18929//PTHR18929:SF99 - PROTEIN DISULFIDE ISOMERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCTCAGCTTGTTGAGCTCCTTGATG |
| Internal bar code: | GGATGATGTCGATGTCAGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 96.5909 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACAGGCTCACAATCAGC |
| Suggested primer 2: | ACAGCACCAGCAGTGTGTTC |